Age-related macular degeneration (AMD) causes central vision impairment with increased incidence. In the pathogenesis of AMD, reactive oxygen species (ROS) are associated with RPE cell apoptosis. H2O2 is an oxidative toxicant and is used to establish the AMD in vitro model. However, the mechanisms of ROS in H2O2-induced AMD are still unclear. Fullerenol, a promising antioxidant of nanomaterials, protects RPE cells from ROS attack. In addition to working as a scavenger, little is known about the antioxidant mechanism of fullerenol in RPE cells. In this study, transcriptome sequencing was performed to examine the global changes in mRNA transcripts induced by H2O2 in human ARPE-19 cells. Moreover, we comprehensively investigated the protective effects of fullerenol against H2O2-induced oxidative injury by RNA sequencing. Gene Ontology enrichment analysis showed that those pathways related to the release of positive regulation of DNA-templated transcription and negative regulation of apoptotic process were affected. Finally, we found that 12 hub genes were related to the oxidative-protection function of fullerenol. In summary, H2O2 affected these hub genes and signaling pathways to regulate the senescence of RPE cells. Moreover, fullerenol is a potent nanomaterial that protects the RPE and would be a promising approach for AMD prevention.
Related researches 71 articles
![<strong>Exploring the World of Fullerenols: A Deep Dive into Their Potential Medical Use</strong>](https://biofullerene.com/wp-content/uploads/2024/03/20-years-research-help-with-oncology-356x356.webp)
![Fullerenol has pronounced antiradical properties in the working concentration range](https://biofullerene.com/wp-content/uploads/2023/06/2021-se-rdm-molecules-all-312x356.png)
![Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents](https://biofullerene.com/wp-content/uploads/2022/12/antioxidant-vector-icon-radical-free-260nw-1596766771.jpg)
![Exploiting the physicochemical properties of dendritic polymers for environmental and biological applications](https://biofullerene.com/wp-content/uploads/2022/12/678-6786008_software-418x356.png)
![Impacts of fullerene derivatives on regulating the structure and assembly of collagen molecules](https://biofullerene.com/wp-content/uploads/2022/12/istockphoto-12085167-356x356.jpg)
![INHIBITORY POTENTIAL OF POLYHYDROXYLATED FULLERENES AGAINST PROTEIN TYROSINE PHOSPHATASE 1B](https://biofullerene.com/wp-content/uploads/2022/12/sol5379-356x356.jpg)
![The neuroprotective effect of fullerenols on a model of Parkinson’s disease in Drosophila melanogaster](https://biofullerene.com/wp-content/uploads/2022/12/PCORI-Story-Women-Pa-314x356.png)
![Effect of fullerenol nanoparticles on oxidative stress induced by paraquat in honey bees](https://biofullerene.com/wp-content/uploads/2022/12/1471354356_medonosny-1-500x293.jpg)
![Facile synthesis of highly water-soluble fullerenes more than half-covered by hydroxyl groups](https://biofullerene.com/wp-content/uploads/2022/12/360_F_308785794_MbgN-500x228.jpg)
![Interaction of fullerenol with lysozyme investigated by experimental and computational approaches](https://biofullerene.com/wp-content/uploads/2022/12/png-clipart-biomolec-500x284.png)
![Effects of hydroxyl group distribution on the reactivity, stability and optical properties of fullerenols](https://biofullerene.com/wp-content/uploads/2022/12/unnamed_1-500x349.jpg)
![Facile synthesis of isomerically pure fullerenols and formation of spherical aggregates from C60(OH)8](https://biofullerene.com/wp-content/uploads/2022/12/images.jpg)
![Influences of the size and hydroxyl number of fullerenes/fullerenols on their interactions with proteins](https://biofullerene.com/wp-content/uploads/2022/12/protein-3jpg57eb1785-356x356.jpg)
![The properties of small fullerenol cluster (C60(OH)24)7: computer simulation](https://biofullerene.com/wp-content/uploads/2022/12/unnamed-3-500x257.jpg)
![The structural studies of fullerenol C60(OH)24 and nitric oxide mixture in water solvent – MD simulation](https://biofullerene.com/wp-content/uploads/2022/12/n-a.jpg)
![Production of monoclonal antibodies against fullerene C60 and development of a fullerene enzyme immunoassay](https://biofullerene.com/wp-content/uploads/2022/12/hd-antibody-blue-485x356.png)
![Mechanism of taq DNA polymerase inhibition by fullerene derivatives: insight from computer simulations](https://biofullerene.com/wp-content/uploads/2022/12/1412-356x356.jpg)
![Polyhydroxylated C60 fullerene (fullerenol) attenuates neutrophilic lung inflammation in mice](https://biofullerene.com/wp-content/uploads/2022/12/11588808685cy47cvm6-412x356.png)
![Morphologically virus-like fullerenol nanoparticles act as the dual-functional nanoadjuvant for HIV-1 vaccine](https://biofullerene.com/wp-content/uploads/2022/12/hiv-356x356.png)
![Fullerenol C₆₀(OH)₃₆ could associate to band 3 protein of human erythrocyte membranes](https://biofullerene.com/wp-content/uploads/2022/12/1043132-356x356.png)
![Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites](https://biofullerene.com/wp-content/uploads/2022/12/medicircle-nanomedic-500x281.jpg)
![Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation](https://biofullerene.com/wp-content/uploads/2022/12/investigation-2-500x333.jpg)
![Self-assembling, reactivity and molecular dynamics of fullerenol nanoparticles](https://biofullerene.com/wp-content/uploads/2022/12/240fed07347d44de5685-356x356.png)
![Fullerenol C60(OH)24 increases ion permeability of lipid membranes in a pH-dependent manner](https://biofullerene.com/wp-content/uploads/2022/12/image3-358x356.png)
![Novel green PVA-fullerenol mixed matrix supported membranes for separating water-THF mixtures by pervaporation](https://biofullerene.com/wp-content/uploads/2022/12/istockphoto-12085167-356x356.jpg)
![Inhalable gadofullerenol/[70] fullerenol as high-efficiency ROS scavengers for pulmonary fibrosis therapy](https://biofullerene.com/wp-content/uploads/2022/12/istockphoto-12925559-440x356.jpg)
![Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards](https://biofullerene.com/wp-content/uploads/2022/12/pngtree-an-icon-sign-356x356.jpg)
![Fullerenol C 60(OH) 36 protects human erythrocyte membrane against high-energy electrons](https://biofullerene.com/wp-content/uploads/2022/12/1-14308-500x281.jpg)
![Molecular Semiconductor Surfactants with Fullerenol Heads and Colored Tails for Carbon Dioxide Photoconversion](https://biofullerene.com/wp-content/uploads/2022/12/71092153-fe1fb500-21-500x343.png)
![Fullerenol Nanoparticles Eradicate Helicobacter pylori via pH-Responsive Peroxidase Activity](https://biofullerene.com/wp-content/uploads/2022/11/701f0ea8629699ea4b87-500x333.jpg)