Gadolinium-containing fullerenol Gd@C82(OH)22#nbsp;has demonstrated low-toxicity and highly therapeutic efficacy in inhibiting tumor growth and metastasis through new strategy of encaging cancer, however, little is known about the mechanisms how this nanoparticle regulates fibroblast cells to prison (instead of poison) cancer cells. Here, we report that Gd@C82(OH)22#nbsp;promote the binding activity of tumor necrosis factor (TNFα) to tumor necrosis factor receptors 2 (TNFR2), activate TNFR2/p38 MAPK signaling pathway to increase cellular collagen expression in fibrosarcoma cells and human primary lung cancer associated fibroblasts isolated from patients. We also employ molecular dynamics simulations to study the atomic-scale mechanisms that dictate how Gd@C82(OH)22#nbsp;mediates interactions between TNFα and TNFRs. Our data suggest that Gd@C82(OH)22#nbsp;might enhance the association between TNFα and TNFR2 through a “bridge-like” mode of interaction; by contrast, the fullerenol appears to inhibit TNFα-TNFR1 association by binding to two of the receptor’s cysteine-rich domains. In concert, our results uncover a sequential, systemic process by which Gd@C82(OH)22#nbsp;acts to prison tumor cells, providing new insights into principles of designs of cancer therapeutics.
Related researches 41 articles
![<strong>Exploring the World of Fullerenols: A Deep Dive into Their Potential Medical Use</strong>](https://biofullerene.com/wp-content/uploads/2024/03/20-years-research-help-with-oncology-356x356.webp)
![Biological and biocompatible characteristics of fullerenols nanomaterials for tissue engineering](https://biofullerene.com/wp-content/uploads/2022/12/photo_2022-12-29_12-06-18-500x317.jpg)
![Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics](https://biofullerene.com/wp-content/uploads/2022/11/ImageForArticle_4620-500x333.jpg)
![Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line](https://biofullerene.com/wp-content/uploads/2022/11/212206-356x356.png)
![Antioxidant properties of fullerenol C60(OH)24 in rat kidneys, testes, and lungs treated with doxorubicin](https://biofullerene.com/wp-content/uploads/2022/11/cancer-icon-2797418_-356x356.png)
![Epigenetic modulation of human breast cancer by metallofullerenol nanoparticles: in vivo treatment and in vitro analysis](https://biofullerene.com/wp-content/uploads/2022/11/targeted-drug-delive-356x356.jpg)
![AFM-based study of fullerenol (C60(OH)24)-induced changes of elasticity in living SMCC-7721 cells](https://biofullerene.com/wp-content/uploads/2022/11/depositphotos_352541-356x356.jpg)
![Fullerenes and their derivatives as inhibitors of tumor necrosis factor-α with highly promoted affinities](https://biofullerene.com/wp-content/uploads/2022/11/0005-009-poluchenie--475x356.jpg)
![Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue](https://biofullerene.com/wp-content/uploads/2022/11/heartAttackCardiacAr-500x346.jpg)
![Hydrophobic Patch of Ubiquitin is Important for its Optimal Activation by Ubiquitin Activating Enzyme E1](https://biofullerene.com/wp-content/uploads/2022/11/1725885-500x263.png)
![Biocompatible [60]/[70] Fullerenols: Potent Defense against Oxidative Injury Induced by Reduplicative Chemotherapy](https://biofullerene.com/wp-content/uploads/2022/11/istockphoto-65584859-356x356.jpg)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/1200px-L-Asparaginsu-500x295.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/5AawkyZS8dY7T9C3AZwH-474x356.jpg)
![Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization](https://biofullerene.com/wp-content/uploads/2022/11/xxx040-512_78628-356x356.png)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/icon-research2x-356x356.png)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/4970457-middle-500x202.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/360_F_337277306_bOwr-500x333.jpg)
![Identification differential behavior of Gd@C 82(OH) 22 upon interaction with serum albumin using spectroscopic analysis](https://biofullerene.com/wp-content/uploads/2022/11/market-research-icon-356x356.png)
![Mono-fullerenols modulating cell stiffness by perturbing actin bundling](https://biofullerene.com/wp-content/uploads/2022/11/pressure-resistance--356x356.jpg)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/Breast-Website-Infog-356x356.png)
![Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A](https://biofullerene.com/wp-content/uploads/2022/11/1rnu_assembly-1-356x356.jpeg)
![Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats](https://biofullerene.com/wp-content/uploads/2022/11/BTOB_Oxidative_Stres-356x356.png)