The very effective anticancer drug doxorubicin (DOX) is known to have cardiotoxic side effects, which could be accompanied by autonomic modulation. Autonomic disbalance might even be an initiating mechanism underlying DOX-induced cardiotoxicity and can be studied noninvasively by the analysis of heart rate variability (HRV). A number of strategies have been assessed to predict chemotherapy-induced cardiac dysfunction while HRV, a potential detecting tool, has not yet been tested. Thus, we aimed to determine the effect of DOX treatment on HRV in a rat model of colorectal cancer. While pretreatment with fullerenol (Frl) acts protectively on DOX-induced cardiotoxicity, we aimed to test the effect of Frl pretreatment on DOX-induced HRV alterations. After the induction of colorectal cancer, adult male Wistar rats were treated with saline (n = 7), DOX (1.5 mg/kg per week, n = 7) or DOX after pretreatment with Frl (25 mg/kg per week, n = 7) for three weeks (cumulative DOX dose 4.5 mg/kg). One week after treatment rats were anaesthetized, standard ECG was measured and HRV was analyzed in time and frequency domain. During autopsy the intestines and hearts were gathered for biochemical analysis and histopathological examination. DOX treatment significantly decreased parasympathetically mediated high-frequency component (p<0.05) and increased the low-frequency component of HRV (p<0.05), resulting in an increased LF/HF ratio (p<0.05) in cancerous rats. When pretreated with Frl, DOX-induced HRV alterations were prevented: the high-frequency component of HRV increased (p<0.01), the low-frequency decreased (p<0.01), LF/HF ratio decreased consequently (p<0.01) compared to DOX only treatment. In all DOX-treated animals, disbalance of oxidative status in heart tissue and early myocardial lesions were found and were significantly reduced in rats receiving Frl pretreatment. Autonomic modulation accompanied the development of DOX-induced cardiotoxicity in rat model of colorectal cancer and was prevented by Frl pretreatment. Our results demonstrated the positive prognostic power of HRV for the early detection of DOX-induced cardiotoxicity.
Related researches 41 articles
![<strong>Exploring the World of Fullerenols: A Deep Dive into Their Potential Medical Use</strong>](https://biofullerene.com/wp-content/uploads/2024/03/20-years-research-help-with-oncology-356x356.webp)
![Biological and biocompatible characteristics of fullerenols nanomaterials for tissue engineering](https://biofullerene.com/wp-content/uploads/2022/12/photo_2022-12-29_12-06-18-500x317.jpg)
![Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics](https://biofullerene.com/wp-content/uploads/2022/11/ImageForArticle_4620-500x333.jpg)
![Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line](https://biofullerene.com/wp-content/uploads/2022/11/212206-356x356.png)
![Antioxidant properties of fullerenol C60(OH)24 in rat kidneys, testes, and lungs treated with doxorubicin](https://biofullerene.com/wp-content/uploads/2022/11/cancer-icon-2797418_-356x356.png)
![Epigenetic modulation of human breast cancer by metallofullerenol nanoparticles: in vivo treatment and in vitro analysis](https://biofullerene.com/wp-content/uploads/2022/11/targeted-drug-delive-356x356.jpg)
![AFM-based study of fullerenol (C60(OH)24)-induced changes of elasticity in living SMCC-7721 cells](https://biofullerene.com/wp-content/uploads/2022/11/depositphotos_352541-356x356.jpg)
![Fullerenes and their derivatives as inhibitors of tumor necrosis factor-α with highly promoted affinities](https://biofullerene.com/wp-content/uploads/2022/11/0005-009-poluchenie--475x356.jpg)
![Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue](https://biofullerene.com/wp-content/uploads/2022/11/heartAttackCardiacAr-500x346.jpg)
![Hydrophobic Patch of Ubiquitin is Important for its Optimal Activation by Ubiquitin Activating Enzyme E1](https://biofullerene.com/wp-content/uploads/2022/11/1725885-500x263.png)
![Biocompatible [60]/[70] Fullerenols: Potent Defense against Oxidative Injury Induced by Reduplicative Chemotherapy](https://biofullerene.com/wp-content/uploads/2022/11/istockphoto-65584859-356x356.jpg)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/1200px-L-Asparaginsu-500x295.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/5AawkyZS8dY7T9C3AZwH-474x356.jpg)
![Molecular mechanism of Gd@C 82(OH) 22 increasing collagen expression: Implication for encaging tumor](https://biofullerene.com/wp-content/uploads/2022/11/4264665-356x356.png)
![Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization](https://biofullerene.com/wp-content/uploads/2022/11/xxx040-512_78628-356x356.png)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/icon-research2x-356x356.png)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/4970457-middle-500x202.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/360_F_337277306_bOwr-500x333.jpg)
![Identification differential behavior of Gd@C 82(OH) 22 upon interaction with serum albumin using spectroscopic analysis](https://biofullerene.com/wp-content/uploads/2022/11/market-research-icon-356x356.png)
![Mono-fullerenols modulating cell stiffness by perturbing actin bundling](https://biofullerene.com/wp-content/uploads/2022/11/pressure-resistance--356x356.jpg)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/Breast-Website-Infog-356x356.png)
![Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A](https://biofullerene.com/wp-content/uploads/2022/11/1rnu_assembly-1-356x356.jpeg)
![Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats](https://biofullerene.com/wp-content/uploads/2022/11/BTOB_Oxidative_Stres-356x356.png)