In this study, the alterations of the morphology and biomechanical properties of living SMCC-7721 cancer cells treated with fullerenol (C60(OH)24) for 24, 48, and 72h were investigated using an atomic force microscope (AFM). Comparative analyses show that the elastic moduli of the SMCC-7721 cells exposed to fullerenol decrease significantly with the increase of the treatment periods. Furthermore, in different phases of the treatment, a global decrease in elasticity is accompanied by cellular morphological changes, and the time-dependent effect of the fullerenol can be observed using AFM and optical microscope. In addition, as the treatment duration increases, the indentation force and depth penetrated into the cell membrane by the AFM tip are in a declining trend. The reduction in the stiffness of the cells exposed to fullerenol could be associated with the disruption of the cellular cytoskeleton network. The investigation indicates that the elastic modulus of single living cells can be a useful biomarker to evaluate the effects of fullerenol or other anticancer agents on the cells and reveal instructive information for cellular dynamic behaviors.
Related researches 41 articles
![<strong>Exploring the World of Fullerenols: A Deep Dive into Their Potential Medical Use</strong>](https://biofullerene.com/wp-content/uploads/2024/03/20-years-research-help-with-oncology-356x356.webp)
![Biological and biocompatible characteristics of fullerenols nanomaterials for tissue engineering](https://biofullerene.com/wp-content/uploads/2022/12/photo_2022-12-29_12-06-18-500x317.jpg)
![Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics](https://biofullerene.com/wp-content/uploads/2022/11/ImageForArticle_4620-500x333.jpg)
![Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line](https://biofullerene.com/wp-content/uploads/2022/11/212206-356x356.png)
![Antioxidant properties of fullerenol C60(OH)24 in rat kidneys, testes, and lungs treated with doxorubicin](https://biofullerene.com/wp-content/uploads/2022/11/cancer-icon-2797418_-356x356.png)
![Epigenetic modulation of human breast cancer by metallofullerenol nanoparticles: in vivo treatment and in vitro analysis](https://biofullerene.com/wp-content/uploads/2022/11/targeted-drug-delive-356x356.jpg)
![Fullerenes and their derivatives as inhibitors of tumor necrosis factor-α with highly promoted affinities](https://biofullerene.com/wp-content/uploads/2022/11/0005-009-poluchenie--475x356.jpg)
![Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue](https://biofullerene.com/wp-content/uploads/2022/11/heartAttackCardiacAr-500x346.jpg)
![Hydrophobic Patch of Ubiquitin is Important for its Optimal Activation by Ubiquitin Activating Enzyme E1](https://biofullerene.com/wp-content/uploads/2022/11/1725885-500x263.png)
![Biocompatible [60]/[70] Fullerenols: Potent Defense against Oxidative Injury Induced by Reduplicative Chemotherapy](https://biofullerene.com/wp-content/uploads/2022/11/istockphoto-65584859-356x356.jpg)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/1200px-L-Asparaginsu-500x295.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/5AawkyZS8dY7T9C3AZwH-474x356.jpg)
![Molecular mechanism of Gd@C 82(OH) 22 increasing collagen expression: Implication for encaging tumor](https://biofullerene.com/wp-content/uploads/2022/11/4264665-356x356.png)
![Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization](https://biofullerene.com/wp-content/uploads/2022/11/xxx040-512_78628-356x356.png)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/icon-research2x-356x356.png)
![Aspartic acid derivatized hydroxylated fullerenes as drug delivery vehicles for docetaxel: an explorative study](https://biofullerene.com/wp-content/uploads/2022/11/4970457-middle-500x202.png)
![Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy](https://biofullerene.com/wp-content/uploads/2022/11/360_F_337277306_bOwr-500x333.jpg)
![Identification differential behavior of Gd@C 82(OH) 22 upon interaction with serum albumin using spectroscopic analysis](https://biofullerene.com/wp-content/uploads/2022/11/market-research-icon-356x356.png)
![Mono-fullerenols modulating cell stiffness by perturbing actin bundling](https://biofullerene.com/wp-content/uploads/2022/11/pressure-resistance--356x356.jpg)
![Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests](https://biofullerene.com/wp-content/uploads/2022/11/Breast-Website-Infog-356x356.png)
![Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A](https://biofullerene.com/wp-content/uploads/2022/11/1rnu_assembly-1-356x356.jpeg)
![Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats](https://biofullerene.com/wp-content/uploads/2022/11/BTOB_Oxidative_Stres-356x356.png)